Advanced tRNA Modification Detection Solutions to Support Genetic Research

0
666

Creative Diagnostics, a leading manufacturer and supplier of antibodies, antigens and assay kits, has announced its comprehensive portfolio of highly validated antibodies for tRNA Modification Detection. These antibody-based analysis solutions provide reliable and sensitive detection methods for the scientific community to identify specific tRNA modifications.

Transfer ribonucleic acid (tRNA) is present in tens of millions of transcripts in human cells and is the most abundant of all cellular RNAs. tRNAs are the most extensively modified RNAs, with an average of 13 modifications per molecule. tRNAs are well known for their primary function as adaptors of amino acids and the genetic code in protein synthesis. tRNA abundance, modification, and aminoacylation (charge) levels contribute to mRNA decoding in a manner that reflects the cell type and its environment; however, how these factors work together to maximize translation efficiency remains to be understood. tRNAs also interact with many proteins that are not involved in translation, which may orchestrate translation activity and other processes in the cell.

Traditional techniques for tRNA modification analysis, such as liquid chromatography-mass spectrometry (LC-MS) and next-generation sequencing (NGS), are often challenged by the complexity of sample handling and preservation of structural context. To address these limitations, Creative Diagnostics offers a promising alternative through its wide range of antibody solutions.

Creative Diagnostics now provides scientists with various tools for accurate quantification and identification of tRNA modifications within complex biological samples, such as the Anti-2'-O-methylcytidine/Cm monoclonal antibody (Catalog # DMABB-JX352), Anti-5-methylcytidine (m5C) monoclonal antibody (Catalog # DMABC-JX060), and Anti-M6A Monoclonal antibody, clone 3I7 (Catalog # CABT-CS610). They are suitable for Dot, ELISA, and IF. In addition, this newly expanded antibody portfolio can support the detection of common modifications, including Methylation, Thiolation, Pseudouridylation, Dihydrouridylation, and Acetylation.

For example, the Rabbit Anti-M6A Monoclonal antibody, clone 3I7 (CABT-CS610) can be applied in ELISA, DB, and IF. N6-methyladenosine (m6A) is the most common and abundant modification in eukaryotic RNA molecules. m6A modification is catalyzed by the methyltransferase complex METTL3 and removed by the recently discovered m6A RNA demethylases FTO and ALKBH5, which catalyze m6A demethylation in an α-ketoglutarate (α-KG)- and Fe2+-dependent manner. Studies have shown that METTL3, FTO and ALKBH5 play critical roles in many biological processes ranging from development and metabolism to reproduction. Additionally, m6A accounts for more than 80% of all RNA base methylation and exists in different species. It is predominantly distributed in mRNAs, but also occurs in non-coding RNAs, such as tRNAs, rRNAs and snRNAs.

Creative Diagnostics offers a comprehensive list of quality antibodies for tRNA modification detection. Researchers interested in learning more about Creative Diagnostics' antibodies and other research tools are encouraged to visit https://www.creative-diagnostics.com/trna-modifications-detection.htm.

About Creative Diagnostics

Creative Diagnostics is a leading manufacturer and supplier of antibodies, viral antigens, innovative diagnostic components, and critical assay reagents. In addition to providing contract R&D and biologic manufacturing services for diagnostic manufacturers along with GMP biologics manufacturing for the biopharmaceutical market, the company aims to continue to act as a trusted source for all researchers' assay development and manufacturing needs.

Search
Categories
Read More
Games
MMOexp BnS NEO Classic Divine Gems: Seasonal Events Offer Bonus Opportunities
Blade & Soul NEO Classic, the remastered version of the acclaimed MMORPG, has recently...
By Tesioao Ddjsi 2025-03-17 01:48:54 0 2K
Games
Poe 2 trade: Where to Farm the Most XP
Path of Exile 2 (PoE 2), an exciting new chapter in the Path of Exile franchise, is a game that...
By Jake Jake 2025-03-28 08:15:26 0 1K
Games
MMOexp BnS NEO Classic Divine Gems Tips for Efficient Progression
In Blade & Soul NEO Classic, Divine Gems are a vital enhancement system that significantly...
By Tesioao Ddjsi 2025-06-20 02:37:21 0 16
Shopping
犀利士官網醫師:犀利士有延時效果?好的生活習慣有助於治療早洩
隨著生活節奏的加快,以及工作壓力的增加,現在很多男性過兩性生活的時間越來越短,於是造成了過兩性生活的不愉快。在這時候,許多男性朋友可能就會想到如何用最簡單的方式來延長夫妻生活。有傳言說犀利士除了...
By James Layne 2025-05-16 06:17:29 0 622
Games
The Best Slayer Tasks to Skip in OSRS gold
In Old School RuneScape (OSRS), Slayer tasks are a major aspect of the game, offering both...
By Jake Jake 2025-04-09 08:55:21 0 1K